m6A sequencing of bladder cancer cells

N6-methyladenosine (m6A) is the most abundant modification in eukaryotic messenger RNAs (mRNAs), and plays important roles in many bioprocesses. However, its functions in bladder cancer (BCa) remain elusive. Here, we discovered that methyltransferase-like 3 (METTL3), a major RNA N6-adenosine methyltransferase, was significantly up-regulated in human BCa and play crucial roles in BCa cell proliferation, invasion, and survival in vitro and tumorigenicity in vivo. Through transcriptome sequencing, m6A sequencing and m6A methylated RNA immuno-precipitation quantitative reverse-transcription polymerase chain reaction, we revealed a systematic profile of METTL3-mediated m6A modification in BCa for the first time.