Nucleome Dynamics during Retinal Development [scRNA-seq/scATAC-seq_Mm]

Single cell RNA-sequencing shows cell type specific expression of genes in the retina. Single cell ATAC-sequencing of the wild type retina revealed that chromatin in the Vsx2 CRC SE, a stage- and cell type-specific core regulatory circuit super-enhancer (CRC-SE) upstream of the Vsx2 gene, was only open in bipolar cells. Deletion of the Vsx2 CRC-SE in mice led to the loss of bipolar neurons in the retina, which was confirmed by scRNA-sequencing of Vsx2SEKO mice. Single cell RNA sequencing from adult WT and VSX2SE KO mouse retina and single cell ATAC-sequencing from WT mouse retina. Please note that the scRNA-seq processed data was generated from multiple samples as indicated in the corresponding sample description field and thus, is linked to the Series records.单细胞RNA测序（single cell RNA-sequencing）可揭示视网膜内基因的细胞类型特异性表达模式。针对野生型（wild type，简称WT）视网膜开展的单细胞ATAC测序（single cell ATAC-sequencing）结果显示，位于Vsx2基因上游的Vsx2 CRC SE——一种兼具阶段与细胞类型特异性的核心调控回路超级增强子（core regulatory circuit super-enhancer，简称CRC-SE）——的染色质仅在视网膜双极细胞中处于开放状态。在小鼠体内敲除Vsx2 CRC-SE会导致视网膜双极神经元缺失，该表型可通过对Vsx2SEKO小鼠开展单细胞RNA测序得到验证。本数据集包含成年野生型与VSX2SEKO小鼠视网膜的单细胞RNA测序数据，以及野生型小鼠视网膜的单细胞ATAC测序数据。请注意：本数据集的scRNA-seq处理后数据由多个样本生成，具体信息可参见对应样本描述字段，且该数据已与系列记录关联。