We develop a universal method to label individual DNA molecules for analyzing diverse types of rare genetic variants, with frequency as low as 4x10-5, using short- or long-read sequencing. It enables
We created a mutator protein, eMutaT7[transition] with targeted in vivo hypermutation in E. coli. To investigate the gene-specific mutagenesis, we sequenced ~3.3 kb DNA around the target gene from cel
As an effective programmable DNA targeting tool, CRISPR-Cas9 system has been adopted in a variety of biotechnology applications. However, the off-target effects, derived from the tolerance towards gui
