The enzyme was preincubated for 5 min at 35°C with the various reagents before addition of the substrate. Each reagent was added at the final concentration as indicated.
aTemperatures corresponding to the two maxima of the unfolding profiles seen in Fig. 6.bNumber of independent experiments.cAverages ± range are given.dExperiments were routinely conducted in 20 mM HEP
Note: Results are shown as mean ± standard deviation with n = 6.1β-ME, β-mercaptoethanol; PMSF, phenyl methyl sulfonyl fluoride; EGTA, ethylene glycol tetra acetic acid; EDTA ethylenediamine tetra ace
