The enzyme was preincubated for 5 min at 35°C with the various reagents before addition of the substrate. Each reagent was added at the final concentration as indicated.
Thermodynamic parameters associated with the thermal unfolding of lysozyme and RNase-A in the presence of different concentrations of urea- NaCl mixture at pH 7.0.
The activity of purified dihydropyrimidinase (without any metal supplement in the culture) toward dihydrouracil was analyzed by the standard assay. Metal ions were added to the reaction mixture for di
