The underlined nucleotides correspond to EcoRI or AatII restriction sites.Names and sequences of oligonucleotides used for dacC cloning in B. subtilis expression vectors.
The location of primers pairs from 1 to 6 is shown in Figures 1 and 2. Lowercase indicate the region that hybridizes to the 5′ or 3′ end of the pKD3 or pKD4 plasmids.
