The location of primers pairs from 1 to 6 is shown in Figures 1 and 2. Lowercase indicate the region that hybridizes to the 5′ or 3′ end of the pKD3 or pKD4 plasmids.
A single upward arrow (��) indicates an enhanced RBS; double arrows (����) indicate a strongly enhanced RBS.aItalic type indicates recognition site of restriction enzymes, SacI or SpeI, used for cloni
