CD8+ T Cells Promote Maturation of Liver-Resident NK Cells via the CD70-CD27 axis

We discovered that LrNK cells exhibit a relatively immature phenotype and impaired cytotoxic capacity in the absence of CD8+ T cells. The provision of CD8+ T cells to Cd8-/- or Rag1-/- mice led to the restoration of LrNK cell maturation LrNK and cNK mRNA profiles of wild type (WT) and CD8-/- mice were generated by deep sequencing, in triplicate, using Illumina本研究发现，在缺乏CD8阳性T细胞的条件下，肝脏驻留自然杀伤细胞（LrNK cells）呈现相对未成熟的表型，且细胞毒功能受损。向Cd8基因敲除（Cd8-/-）或Rag1基因敲除（Rag1-/-）小鼠输注CD8阳性T细胞，可恢复LrNK细胞的成熟状态。本研究通过Illumina测序平台，对野生型（WT）及Cd8-/-小鼠的LrNK细胞与经典自然杀伤细胞（cNK cells）的mRNA转录组谱完成了三次生物学重复的深度测序。