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RNA-seq using mouse Neuro-2A (N2A) neuroblastoma cells

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NIAID Data Ecosystem2026-03-13 收录
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To identify high-confidence NMD targets in mouse N2A neuroblastoma cells, we used our established transcriptome-wide RNA sequencing (RNA-seq) methodologies. Through parallel analyses of RNA-seq upon UPF1-knockdown (KD) and RNA immunoprecipitation (RIP-seq) footprinting of p-UPF1-bound RNAs, we identified 1027 high-confidence neuronal NMD targets. RNA profiles of N2A cells treated with control siRNA (siCtl; n=2) or Upf1 siRNA (siUpf1; n=3).

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2021-11-30
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