RNA-Sequencing Analysis of of heart tissue from murine model of endotoxemia

Cardiac dysfunction, an early complication of sepsis and endotoxemia, is the major cause of death in intensive care units. To explore the key mechanism in the “double-hit” endotoxemia heart, we performed bulk RNA sequencing and analyses of heart tissue from healthy C57BL/6J mice with endotoxin exposure at early or late stage. The RNA-seq analyses revealed the canonical and non-canonical inflammasome activation is involved in endotoxin-induced cardiac injury. Overall design: Bulk RNA sequencing of heart tissue from healthy C57BL/6J mice (n=6, eight weeks-age) at baseline, after 7-hour poly(I:C) priming,