Transcriptome analysis of gene expression in sec59-1∆ yeast cells

Microarray data revealed that, 1880 genes were up regulated and 1430 genes were down regulated among that 3310 genes, 92 lipidomic genes were up regulated and 17 genes were down regulated in sec59-1∆. In sec59-1∆ cells, phospholipid, neutral lipid, sphingolipid and fatty acid metabolism genes were up regulated. N-Glycosylation defect affected various organelle functions such as mitochondria, peroxisome, vacuole and ER. Finally we can conclude from the microarray data, N-glycosylation plays a crucial role in regulating the lipidome homeostasis Microarray data revealed that, 1880 genes were up regulated and 1430 genes were down regulated among that 3310 genes, 92 lipidomic genes were up regulated and 17 genes were down regulated in sec59-1∆. In sec59-1∆ cells, phospholipid, neutral lipid, sphingolipid and fatty acid metabolism genes were up regulated. N-Glycosylation defect affected various organelle functions such as mitochondria, peroxisome, vacuole and ER. Finally we can conclude from the microarray data, N-glycosylation plays a crucial role in regulating the lipidome homeostasis基因芯片（Microarray）分析结果显示，在sec59-1Δ突变体的3310个检测基因中，共有1880个基因发生上调、1430个基因发生下调；其中92个脂质组相关基因（lipidomic genes）上调，17个基因下调。在sec59-1Δ细胞中，磷脂、中性脂质、鞘脂及脂肪酸代谢相关基因均发生上调。N-糖基化（N-Glycosylation）缺陷会影响线粒体（mitochondria）、过氧化物酶体（peroxisome）、液泡（vacuole）及内质网（ER）等多种细胞器的功能。基于上述基因芯片数据，我们可得出结论：N-糖基化在调控脂质组稳态（lipidome homeostasis）中发挥关键作用。 基因芯片（Microarray）分析结果显示，在sec59-1Δ突变体的3310个检测基因中，共有1880个基因发生上调、1430个基因发生下调；其中92个脂质组相关基因（lipidomic genes）上调，17个基因下调。在sec59-1Δ细胞中，磷脂、中性脂质、鞘脂及脂肪酸代谢相关基因均发生上调。N-糖基化（N-Glycosylation）缺陷会影响线粒体（mitochondria）、过氧化物酶体（peroxisome）、液泡（vacuole）及内质网（ER）等多种细胞器的功能。基于上述基因芯片数据，我们可得出结论：N-糖基化在调控脂质组稳态（lipidome homeostasis）中发挥关键作用。