Sensitivity of the RNA profiling methods using quantitative approach (input totalRNA) by real-time PCR.
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Total RNA was extracted from samples from 4–16 donors (as described in 2.2.1). A range of input total RNA was reverse transcribed and the resulting cDNA was amplified. Each experiment was carried out 3 times. dCt≤23 represent positive results. NT: not tested.
创建时间:
2015-12-02



