Virus neutralizing activity of immune sera from SPF chickens following vaccination with either VRPs, inactivated virus, or live attenuated virus.

aSPF chickens were immunized with four different vaccines.VSV*ΔG(HAH5-HP) and VSV*ΔG(HAH5-LP) were applied intramuscularly without adjuvant using 2×108 ffu for both the primary and booster immunization. Inactivated A/duck/Hokkaido/Vac-1/04 (H5N1) was formulated with adjuvant and applied two times via the intramuscular route. Live A/duck/Hokkaido/Vac-1/04 (H5N1) was applied one time via the intratracheal route using 107 TCID50. Serum was prepared from 4 to 5 animals per group and pooled. Virus neutralization tests were performed with the viruses listed in the left column. Neutralization titers obtained with homologous virus are underlined.