Transcriptional profile of T, ITNK and NK cells

To further characterize the global gene expression profiles of ITNKs, we conducted RNA-sequencing analysis of T cells, ITNKs (CD3+NKp46+) and NK cells (CD3-CD56+) (both the CB and the PBMC origins with > 90% purity) from the CB and PBMC . Principal component analysis (PCA) and unsupervised hierarchical cluster analysis revealed that ITNKs had transcriptomic features of both T cells and NK cells mRNA from sorted T, ITNK and NK cells was prepared according to the TruSeqTM RNA Sample Preparation Guide, and sequencing was performed on a BGISEQ-500