Single-cell sequencing of E12.5 mouse placenta

In our study, we performed single-cell sequencing of the E12.5 mouse placenta to explore the cellular diversity and gene expression dynamics during placental development. This approach allowed us to delineate the complex landscape of cell types and states present at this critical stage of gestation. By analyzing the transcriptomic data, we identified several distinct trophoblast subpopulations, each characterized by unique gene expression patterns that suggest different functional roles in placental structure and function. Additionally, our data provided insights into the potential regulatory pathways involved in cell differentiation. We utilize placental samples from E12.5 mice, specifically choosing two distinct genotypes: wild-type (control group) and Elf5-Cre; Slc25a1f/f (experimental group). Wild-type placentas serve as control samples to evaluate the impact of Slc25a1 deletion on placental development.本研究针对妊娠第12.5天（E12.5）的小鼠胎盘开展单细胞测序，旨在探究胎盘发育过程中的细胞多样性与基因表达动态变化。该测序策略得以让我们清晰勾勒出这一妊娠关键阶段中存在的各类细胞类型与细胞状态的复杂图谱。通过对转录组数据的分析，我们鉴定出多个特征鲜明的滋养层细胞亚群，每个亚群均具备独特的基因表达谱，提示其在胎盘结构构建与功能行使中承担着不同的生物学功能。此外，本研究数据为解析细胞分化过程中潜在的调控通路提供了全新视角。本研究采用E12.5小鼠的胎盘样本，特意选取两种不同基因型的个体：野生型（对照组）与Elf5-Cre; Slc25a1f/f（实验组）。野生型胎盘作为对照样本，用于评估Slc25a1基因敲除对胎盘发育的影响。