Nucleotide sequences of primer pairs, product length and specific annealing temperatures (Ta) applied for the real-time reverse transcriptase polymerase chain reaction (RT- PCR) detection.
Primer melting temperature is given under (as calculated by the wEMBOSS [57] program ‘dan’). ‘Length’ denotes the length of the amplicon in basepairs, ‘Reference’ indicates from which publication the
Am: amplicon size. Bp: number of base pairs. Tm: melting temperature. RT: retro-transcription primer. R: reverse primer. F: Forward primer. Ref: references/PrimerBankID/P3: primers were designed using