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Description of gene-specific real-time PCR assays.

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Am: amplicon size. Bp: number of base pairs. Tm: melting temperature. RT: retro-transcription primer. R: reverse primer. F: Forward primer. Ref: references/PrimerBankID/P3: primers were designed using Primer3 software. C: concentration of primers in qPCR reaction in nM. RNA input: respective amount of RNA in qPCR reaction in ng (in brackets: if different amount for small intestine samples were used). E: Assays efficiency.

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2014-01-17
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