DEtail-seq profiled meiotic DSBs in Saccharomyces cerevisiae

SPO11-promoted DNA double-strand breaks (DSBs) formation is a crucial step for meiotic recombination, and it is indispensable to detect the broken DNA ends accurately for dissecting the molecular mechanisms behind. Here, we report a novel technique, named DEtail-seq (DNA End tailing followed by sequencing), that can directly and quantitatively capture the meiotic DSB 3’ overhang hotspots at single-nucleotide resolution.SPO11介导的DNA双链断裂（double-strand breaks, DSBs）的形成是减数分裂重组的关键步骤，精准检测断裂DNA末端对于解析其背后的分子机制不可或缺。本研究报道了一种新型技术，命名为DEtail-seq（DNA末端加尾后测序，DNA End tailing followed by sequencing），该技术可实现单核苷酸分辨率下对减数分裂DSB的3'突出端热点区域的直接定量捕获。