Tead1 regulation of beta-cells

Purpose: The goal of this study is to determine the regulatory role of tead1 in beta-cells by analyzing the transcriptomal changes with Tead1 deletion in beta-cells Methods: Isolated islet mRNA profiles of beta-cell Tead1 KO mice compared to control floxed mice at 1 year of age were assessed by RNA-seq using Illumina Hiseq2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level using the CLC genomic workbench. qRT-PCR validation was performed using SYBR Green assays Conclusions: Our study represents the first detailed analysis of beta cell transcriptomes following Tead1 deletion in beta cells. Isolated islet total RNA was used for library prep from 3 pooled samples for each of 2 samples for each genotype.研究目的：本研究旨在通过分析β细胞中Tead1敲除后的转录组变化，明确Tead1在β细胞中的调控作用。 实验方法：本研究采用Illumina Hiseq2500平台进行RNA测序（RNA-seq），对1年龄的β细胞特异性Tead1敲除（KO）小鼠与对照floxed小鼠的分离胰岛mRNA表达谱进行对比分析。经质量过滤的测序读段，通过CLC基因组工作台（CLC genomic workbench）在转录本亚型层面完成数据分析。此外，采用SYBR Green染料法开展qRT-PCR验证实验。 研究结论：本研究首次对β细胞中Tead1敲除后的胰岛转录组进行了详细解析。本实验中，每种基因型设置2份样本，每份样本的总RNA取自3个混合的分离胰岛，用于文库构建。