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m6A binding proteins identified by SPIDER assay

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国家人口健康科学数据中心2026-06-01 收录
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https://www.ncmi.cn/phda/dataDetails.do?id=CSTR:17970.11.A001G.202308.2134.V1.0
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To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding proteins within a total cell lysate. We performed SPIDER assay by incubating biotin-ssRNAs(with and without m6A modification)with the total lysate of YTDHFs (YTHDF1,YTHDF2 and YTHDF3)-overexpressed HEK293T cells
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2022-11-02
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