To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding protei
To assess whether SPIDER could capture protein-small molecule interaction in a complex environment, here we carried out the SPIDER assay by incubating biotin-Lenalidomide or a biotin control with HEK2
We identified the major RNA binding protein-SFPQ as a direct interaction partner of FTO. Our study showed that FTO and SFPQ were located in close proximity throughout the transcriptome and overexpress