Translation dysregulation in cancer as a source for targetable antigens.

We used MULTI-seq (McGinnis, Patterson, et al., Nature Methods, 2019) to perform multiplexed single-cell RNA-sequencing analysis of cryopreserved primary melanoma tumor samples isolated from mice inoculated with TYW2 WT or KO tumors. Presented data represent CD45+ immune cells isolated from both tumor backgrounds at two timepoints (D18 and D21). Overall design: MULTI-seq barcoded CD45+ mouse immune cells were isolated using FACS and analyzed using 3' scRNA-seq (10x Genomics)