Effect of Tet2 deletion on gene expression in mice

To investigate the effect of Tet2 on gene expression, we established Nestin Cre+ Tet2lox/lox (Tet2 cKO) mice and Nestin Cre- Tet2lox/lox (WT) mice , collected their habenular tissue, and compared the differential gene expressions at mRNA levels. We then performed gene expression profiling analysis using data obtained from RNA-seq in the habenular of 2-month mice. Comparative gene expression profiling analysis of RNA-seq data in the habenular of Tet2 cKO mice and WT mice, n=3.为探究Tet2对基因表达的影响，我们构建了巢蛋白（Nestin）Cre重组酶阳性且Tet2基因loxP位点纯合的Tet2条件性敲除（Tet2 cKO）小鼠，以及巢蛋白Cre重组酶阴性且Tet2基因loxP位点纯合的野生型（Wild Type, WT）小鼠，收集两组小鼠的缰核组织，在mRNA水平上比较其差异基因表达情况。随后，我们使用2月龄小鼠缰核的RNA测序（RNA-seq）数据开展比较基因表达谱分析，针对Tet2 cKO小鼠与WT小鼠的缰核转录组数据进行相关分析，每组生物学重复数n=3。