Context dependent function of transcriptional regulator Rap1 in gene silencing and activation [ChIP-seq III]

As part of a study on the mechanism of Sir-based silencing at HML in S.cerevisiae, and the contribution of Rap1 to transcription of the locus, we performed ChIP-seq of multiple components of RNA Pol II machinery in different genetic backgrounds including: sir4∆, SIR+, and with or without a mutation that abbrogated Rap1 binding at the alpha promoter.本研究围绕酿酒酵母（Saccharomyces cerevisiae）中HML位点依赖Sir蛋白的沉默机制，以及Rap1对该基因座转录的调控贡献展开，在多种遗传背景下对RNA聚合酶II（RNA Pol II）复合物的多个组分进行了染色质免疫共沉淀测序（ChIP-seq）。所涉及的遗传背景包括：sir4∆缺失突变体、SIR+野生型菌株，以及携带或不携带可阻断Rap1与α启动子结合突变的菌株。