The effect of Lats2 knockout and diet on liver disease in mice

Liver samples of mice harboring liver-specific deletion of Lats2 (Lats2-CKO) were compared to WT mice. This effect was tested along with the effect of diet - high cholesterol diet (Paigen-PD) vs. normal chow (ND). Expression array analysis on Affymetrix microarrays was carried out for livers from WT and Lats2-CKO mice fed ND or HCD for 9 weeks.将肝脏特异性敲除Lats2基因（Lats2-CKO）的小鼠的肝脏样本与野生型（Wild Type, WT）小鼠进行对比。本研究同时验证了该基因敲除模型的效应与饮食因素的效应，其中饮食因素设置为帕金高胆固醇饲料（Paigen-PD）与普通维持饲料（Normal Chow, ND）的对照。针对喂食普通维持饲料或高胆固醇饲料达9周的野生型与Lats2-CKO小鼠的肝脏组织，本研究采用Affymetrix微阵列芯片开展了基因表达阵列分析。