RNA-seq of paired oocytes and granulosa cells from 3M and 12M old mice after natural and superovulation

Oocytes and granulosa cells were collected after natural and superovulation in young (3 month-old) and old (12 month-old) mice while conserving pairing information between oocyte and granulosa cells from each cumulus-oocyte-complex (COC). This experimental design allows for the analysis of the effects of ageing and / or superovulation on the transcriptome of COCs. The naming convention for samples is as follows: [ovulation, NO/SO][age, 3M/12M][cell, OC/GC][COC number], meaning that NO12MOC01 and NO12MGC01 are from the same COC. Oocyte and granulosa cell samples were processed for full-length cytoplasmic RNA amplification using slightly modified SMART-seq2 protocol. After amplification of cDNA and library preparation, samples were sequenced on NextSeq 550 using Single-Ended 75bp High-Output kit.我们从年轻（3月龄）与年老（12月龄）小鼠的自然排卵及超排卵处理后收集卵母细胞与颗粒细胞，并保留了每个卵丘卵母细胞复合体（cumulus-oocyte-complex, COC）中卵母细胞与颗粒细胞的配对关联信息。本实验设计可用于探究衰老与/或超排卵对卵丘卵母细胞复合体转录组的影响。样本命名规范如下：[排卵方式，自然排卵（NO）/超排卵（SO）][年龄，3M/12M][细胞类型，卵母细胞（OC）/颗粒细胞（GC）][COC编号]，其中NO12MOC01与NO12MGC01即源自同一COC。我们采用经小幅改良的SMART-seq2方案，对卵母细胞与颗粒细胞样本开展全长细胞质RNA扩增。完成cDNA扩增与文库制备后，使用NextSeq 550测序平台搭配单端75bp高输出试剂盒完成样本测序。