Neferine treatment lower blood pressure in SHR

The goals of this study is to compare the differently expressed genes in abdominal aorta tissue of WKY and SHR as well as differently expressed genes in the abdominal aorta tissue of SHR with or without neferine treatment. The rat (n=15) were randomly divided into 3 groups: WKY,SHR, and SHR + neferine - H (high concentration) groups (n=6 for each group). Rat in WKY and SHR groups were intragastrically with double distilled water (dd H2O); while rat in SHR + SHR + neferine - H groups were intragastrically with 10mg/kg/D of neferine for 10 weeks. Then the abdominal aorta were used to identify differentially expressed genes among different groups.本研究旨在比较Wistar-Kyoto大鼠（WKY）与自发性高血压大鼠（SHR）腹主动脉组织的差异表达基因（differentially expressed genes），以及经莲心碱（neferine）处理与未处理的SHR大鼠腹主动脉组织的差异表达基因。将15只大鼠随机分为3组：WKY组、SHR组及SHR+莲心碱高剂量组（neferine - H，high concentration），每组6只。其中WKY组与SHR组大鼠以双蒸水（dd H₂O）灌胃给药；SHR+莲心碱高剂量组大鼠以10mg/kg/日的莲心碱灌胃给药，持续10周。随后采集各组大鼠腹主动脉组织，用于鉴定不同组间的差异表达基因。