Multi-organ Gene Expression Dynamics and Network Modeling Reveals Sex-specific Shifts in Regulatory Control during the Development of Hypertension

In this study we performed high throughput microfluidic qPCR using BioMark on RNA extracted from 5 organs (adrenal gland, kidney, liver, lung, and spleen) at 5 different time points throughout the development of hypertension (8, 10, 12, 16, and 24 weeks) in Spontaneously Hypertensive Rats (SHR) as compared to Wistar-Kyoto (WKY) control in female subjects (n=3).本研究以雌性自发性高血压大鼠（Spontaneously Hypertensive Rats, SHR）为模型，以Wistar-Kyoto（WKY）大鼠作为对照（两组受试样本量均为n=3），在高血压病程的5个不同时间节点（8、10、12、16及24周），分别采集两组大鼠的肾上腺、肾脏、肝脏、肺脏及脾脏5种器官的RNA，采用BioMark平台开展高通量微流控定量PCR（qPCR）检测。