Transcript RNA collected from microglia cultured in the presence of B. burgdorferi (Bb) was compared to that of RNA collected from microglia (μG) cultured in medium alone. RT-PCR reactions were run in
Mean of the cycle threshold (Ct) and their standard error (SE) for the eight reference genes in different organs (A and B) under various conditions (C, D, E and F) as well as whole dataset (G).