Confirmation of rhesus array results using QRT-PCR.
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Transcript RNA collected from microglia cultured in the presence of B. burgdorferi (Bb) was compared to that of RNA collected from microglia (μG) cultured in medium alone. RT-PCR reactions were run in triplicate with GAPDH gene expression used as the reference. PCR efficiencies, average fold change and statistics were evaluated using REST© software.
创建时间：
2009-11-13
