Identification and characterization of artery and vein enhancers in the human genome

Our goal was to identify artery- and vein-specific cis regulatory elements in the human genome and use these to determine novel mechanisms that regulate arteriovenous differentiation Artery and vein enhancers were identified using ChIP-Seq for K27ac and EP300 on HUAEC and HUVEC, performed in duplicate. Further analysis included RNAseq in triplicate from the same cell types. To assess binding of known transcription factors NR2F2 and ERG, we performed ChIP-Seq in duplicate samples. We assessed K27ac and K27me3 occupancy under different experimental conditions using triplicate samples from HUVEC