Transcriptome differences between white and brown adipose tissue from wildtype and uncoupling protein 1 knockout mice exposed to different diets and ambient temperatures

We analyzed transcript abundance in interscapular brown and inguinal white adipose tissue of wildtype and UCP1-KO mice either adapted to 20°C or 30°C and fed a high fat or control diet. Male C57BL/6J mice of wildtype or Ucp1-KO genotype were fed a high fat diet (48% energy from fat) or control diet (13% energy from fat) at thermoneutrality (30°C). Control diet was also fed at 20°C, resulting in a total of 6 groups of 8 animals each. After 4 weeks, we prepared interscapular brown adipose tissue and inguinal white adipose tissue, resulting in 96 samples. Of these, we sequenced total RNA and determined transcript abundance.我们对适应20℃或30℃环境、饲喂高脂饮食或对照饮食的野生型（wildtype）与UCP1基因敲除（UCP1-KO）小鼠的肩胛间棕色脂肪组织（interscapular brown adipose tissue）及腹股沟白色脂肪组织（inguinal white adipose tissue）的转录本丰度（transcript abundance）进行了分析。选取携带野生型或Ucp1基因敲除基因型的雄性C57BL/6J小鼠，在热中性温度（thermoneutrality，30℃）环境下分别饲喂高脂饮食（脂肪供能占比48%）与对照饮食（脂肪供能占比13%）；同时在20℃环境下饲喂对照饮食，最终共设置6组实验，每组包含8只动物。饲养4周后，采集肩胛间棕色脂肪组织与腹股沟白色脂肪组织，共获得96份样本。随后对所有样本的总RNA（total RNA）进行测序，以测定其转录本丰度。