Data From: Investigating the stress response of blue mussels to heat tolerance in a Northeast Atlantic hybrid zone

Description of the data and file structure This repository contains the processed datasets used for the ΔCt, ΔΔCt calculations and the Linear Mixed-Effects Models (LMMs) presented in the associated publication. The datasets were derived from RT-qPCR gene expression data by averaging the Ct values of technical replicates for each gene (Assay) within each biological sample (Sample_ID), as described in the Materials and Methods section of the article. Metadata associated with each biological sample include treatment condition (Control or Heat), sampling time point (T0, T1, T2 and T3), genotype (M. edulis, M. galloprovincialis, or admixed/"hybrid"), and experimental tank replicate (C1, C2, C3, H1, H2 and H3). File: Cariolato_et_al_2026_AquacultureReports_RawData_dCt_ddCt.xlsx Description: The dataset "Cariolato_et_al_2026_AquacultureReports_RawData_dCt_ddCt" contains the processed Ct values and associated metadata used to calculate ΔCt and ΔΔCt values. Variables Treatment (Control or Heat) TimePoint (T0, T1, T2, T3) Sample_ID (biological sample unique code) Assay (genes presented in the study: ACT, CYP-A, HSP24, HSP90, HSC71, SOD) mean_Ct: average of Ct values of technical replicates for each assay within each biological sample sd_Ct: standard deviation of mean_Ct Genotype: M. edulis, M. galloprovincialis, Hybrid (admixed ancestry) File: Cariolato_et_al_2026_AquacultureReports_LMM_Dataset.xlsx Description: The dataset "Cariolato_et_al_2026_AquacultureReports_LMM_Dataset" contains the processed dataset used for the Linear Mixed-Effects Model analyses presented in the manuscript. Variables Treatment (Control or Heat) TimePoint (T0, T1, T2, T3) Sample_ID (biological sample unique code) Genotype: M. edulis, M. galloprovincialis, Hybrid (admixed ancestry) Tank (C1, C2, C3, H1, H2, H3) ACT_Ct: average of Ct values of technical replicates for ACT assay within each biological sample CYP-A_Ct: average of Ct values of technical replicates for CYP-A assay within each biological sample HSP24_Ct: average of Ct values of technical replicates for HSP24 assay within each biological sample HSC71_Ct: average of Ct values of technical replicates for HSC71 assay within each biological sample HSP90_Ct: average of Ct values of technical replicates for HSP90 assay within each biological sample SOD_Ct: average of Ct values of technical replicates for SOD assay within each biological sample Code/software These excel files can me imported in RStudio using the function "read_excel" from the "readxl" R package. Access information Data was derived from the following sources: Primer pairs for RT-qPCR of the assay employed in this study were retrieved from the following published papers (as correctly reported in the manuscript) Hsp90: Nielsen et al. 2021 Hsp24: Nielsen et al. 2021 Sod: Nielsen et al. 2021 Hsc71: Place et al. 2008 Act: Salatiello et al. 2022 CYP-A: Salatiello et al. 2022