Postnatal epigenetic development of mouse intestinal stem cells (RNA-seq)

We used RNA sequencing to quantify the gene expression levels in the intestinal stem cells (ISCs) or their progeny during the suckling period of mouse colon development. Lgr5-EGFP mice were used to identify ISC populations in the colons. RNA sequencing was performed using EGFP labeled Lgr5+ ISCs and epithelial cell adhesion molecule (EpCAM) labeled epithelial cells isolated at the beginning and end of the suckling period (postnatal day 0-P0 and P21).本研究采用RNA测序（RNA sequencing）技术，定量分析小鼠结肠发育哺乳期内肠道干细胞（intestinal stem cells，ISCs）及其子代细胞的基因表达水平。实验利用Lgr5-EGFP小鼠鉴定结肠中的肠道干细胞群；于哺乳期起始与结束阶段，即出生后第0天（P0）与出生后第21天（P21），分别分离得到经EGFP标记的Lgr5+肠道干细胞以及经上皮细胞黏附分子（epithelial cell adhesion molecule，EpCAM）标记的上皮细胞，并对上述细胞开展RNA测序。