Protective role of oleic acid in angiotensin II-induced cardiac remodeling

To gain mechanistic insights into the protective effects of oleic acid (OA) on angiotensin II(AngII)-induced cardiac remodeling, a whole transcriptomic analysis of heart were performed using RNA-seq.Comparing with heart of normal mice, a total of 1468 genes (987 upregulated and 481 down regulated) were differentially expressed (FDR<0.05) in heart of AngII-induced mice. Comparing with heart of AngII-induced mice, a total of 785 genes (162 upregulated and 623 down regulated) were differentially expressed (FDR<0.05) in heart of OA treated mice. Importantly, among the 987 upregulated genes in heart of AngII-induced mice,388 genes were significantly reversed in heart of OA treated mice. Overall design: 15 samples. There are 3 groups: Veh_H group, mice were treated with saline. A_H group, mice were subcutaneously implanted AngII pump (1000ng/kg/min). A_OA group, mice were ere subcutaneously implantated AngII pump (1000ng/kg/min) and treated with OA (160mg/kg,0.3mL/mice per day). Heart were isolated and RNA sequencing.