High-throughput error corrected Nanopore single cell transcriptome sequencing

E18 mouse brain single cell profiling using the 10x Genomics Chromium instrument workflow with either Illumina short read sequencing for the standard gene profiling and Nanopore PromethION long read sequencing for isoform profiling. E18 mouse brain single cell Chromium emulsion was split into two aliquots targeting 190 and 951 cells and processed in parallel for gene and isoform profiling with Illumina and Nanopore sequencing.本数据集采用10x Genomics Chromium系统实验流程，对E18小鼠大脑开展单细胞表征分析 (single cell profiling)：其中标准基因表达谱分析采用Illumina短读长测序 (short read sequencing) 技术，异构体谱分析 (isoform profiling) 则使用Nanopore PromethION长读长测序 (long read sequencing) 技术。实验中将E18小鼠大脑单细胞Chromium微乳液体系分为两份等分试样，分别预设捕获190与951个细胞，并通过并行处理，分别完成基于Illumina测序的基因谱分析与基于Nanopore测序的异构体谱分析。