To investigate whether recover DNMT3A expression permitted adult beta cell of ÃRapKO establishing proper transcriptome
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资源简介：
Murine islets were isolated from 8-week-old WT and ÃRapKO mice and initially recovered in 1640 RPMI supplemented with 10% serum, 11.1mM glucose overnight. Then islets were transfected with Lenti-GFP or Lenti-Dnmt3a for 24 hours, following cultured in 1640 RPMI medium for 48 hours.Islets were harvested and stored in -80°C for preparation for RNA sequencing. Overall design: Transcriptome of three groups: WT, ÃRapKO, ÃRapKO+LvDnmt3a
创建时间：
2019-06-02
