RNA-seq transcriptonal profiling in neonatal rat ventricular myocytes under hypertrophic growth

Heart failure is a leading cause of death in US. Hypertension is one of the most important risk factor of heart failure. In the presence of high blood pressure, the heart manifests hypertrophic growth to ameliorate ventricular wall stress. This once adaptive response may progress into decompensation and heart failure. The precise mechanisms governing this transition remain elusive. Here, we aimed to identify novel signaling pathways in cardiac hypertrophic growth. Primary neonatal rat ventricular myocytes (NRVMs) were isolated from 1-2 days old rats and treated with phenylephrine or IGF-1. Total RNA was isolated for RNA-seq analysis.心力衰竭是美国人群的首要致死病因之一。高血压是心力衰竭最重要的危险因素之一。当血压升高时，心脏会发生肥厚性生长以缓解心室壁所承受的应力。这一最初的适应性代偿反应可进展为失代偿状态并最终引发心力衰竭。目前，调控这一病理转变的确切机制仍不明晰。本研究旨在探明心脏肥厚性生长过程中的新型信号通路。我们从1至2日龄大鼠体内分离得到原代新生大鼠心室肌细胞（neonatal rat ventricular myocytes, NRVMs），并分别用苯肾上腺素（phenylephrine）与胰岛素样生长因子1（IGF-1）进行处理，随后提取总RNA用于RNA测序（RNA-seq）分析。