Confirmation of Affymetrix data by real-time RT-PCR for cellular target genes repressed by EBNA-3A.

aUnique gene identification number (ID) according to the National Center for Biotechnology Information (NCBI). bAs defined by GO-term and/or Refseq (NCBI). cx-fold repression was calculated from mean expression values of wt and EBNA-3A negative LCLs originating from the Affymetrix gene array hybridization. dx-fold repression according to real-time RT-PCR data was calculated from mean expression values of 2 independent RNA samples derived from a wt and an EBNA-3A negative LCL, respectively, which were established from donor 3. Note that LCLs from donor 3 were already examined by the Affymetrix screen and represent long-term established B cell lines. ex-fold repression according to real-time RT-PCR data was calculated from mean expression values of 2 independent RNA samples derived from a wt and an EBNA-3A negative LCL, respectively, which were established from donors 6 and 7. Note that LCLs from donors 6 and 7 represent freshly established LCLs and were analyzed 6 weeks p.i.. fminimum x-fold repression was estimated for genes that were down-regulated to levels below detection limits by setting the value to a minimal transcript level detected for the gene.