Blood genomic DNA was extracted using QIAamp DNA Blood Kit (Qiagen), and prepared for WGS using TruSeq Nano DNA HT Sample prep Kit (Illumina) according to manufacturer’s protocols.A single sequencing
(A) The previously published pPax8-rtTA plasmid [7] is shown in the top panel. The CreERT2 coding sequence followed by a polyadenylation signal (pA) was used to replace the rtTA sequence of pPax8-rtTA
Microarray analysis of liver tissue from WT SIRT6 and conditional knockout of SIRT6 using albumin-Cre (SIRT6Co/Co ;Alb-Cre) at 2 and 8 months of age RNA was extracted from mouse liver tissue at 2 and