Next Generation Sequencing assesses 53BP1-regulated gene expression profiling in hTRET-RPE1 cells

The goal of this experiment is to analyze global gene expression profiles after depletion of 53BP1 Overall design: hTERT-RPE1 53BP1 knock-out cell line used in this study were generated using CRISPR-Cas9 gene editing approach. Cell were transduced with a lentiviral expression vector encoding a single guide RNAs (gRNAs) against 53BP1, and cells expressing the vector containing only Cas9, but no sgRNA, served as control cell lines.