Assays with recombinant proteins: Formation of borneol, 5-ketocamphor and 5-exo- hydroxy camphor under various conditions.

Values are the average of 4 replicates ± S.E. 50 mM potassium phosphate buffer (pH 7.4) was used for all the assays. Experimental details are included in Material S1.ND = Not Detected; N/A = Not Applicable.1The reaction mixture contained recombinant P450cam, PdR and PdX and NADH. Oxygen (99%) was bubbled into the buffer for 60 seconds before the assay. The 4e− uncoupling was calculated by taking the difference between the total NADH required and observed. 2 The reaction mixture contained recombinant P450cam, PdR, PdX, and NADH. Air (charcoal filtered) was bubbled into the buffer before the assay. 3 The assay was performed using recombinant P450cam and m-CPBA as a shunt agent. 4 The buffer was sparged with argon (99%). 5 The buffer was treated with oxygen (99% pure, Sigma Aldrich) and assays were performed using camphor.