SARS-CoV-2 Whole Genome Sequencing and assembly
收藏NIAID Data Ecosystem2026-03-13 收录
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资源简介：
cDNA from viral RNA of SARS-CoV-2 was subjected to targeted amplification by PCR. Sequencing was performed on MinION MK1B using SpotON flow cell (FLO-MIN106) of Oxford Nanopore Technology. Amplified DNA were subjected to barcoding and adapter ligation using either the rapid barcoding MIDNIGHT protocol or native barcoding ARTIC protocol. Base-calling and de-multiplexing of Nanopore raw data were performed by Guppy (v.5.0.17) tool.
创建时间：
2022-03-23
