Local states of chromatin compaction in HepG2 cells. Local states of chromatin compaction in HepG2 cells

We reported chromatin compaction states within several nucleosomes in HepG2 cells. To assess the local compaction, fromaldehyde-treated chromatin was appled to fractionation via a sedimentation velocity cetrifugation. While less-crosslinked nucleosomes remained in upper fractions as open chromatin, well-crosslinked nucleosomes were sedimented to lower fractions as compact chromatin. Overall design: NGS analyses of DNA flagments prepared from the uppermost and the lowermost fractions of the chromatin fractionation.