Endothelial Mekk3 alters fates of embryonic arterial endothelial cells

To examine how endothelial Mekk3 affects the cell and molecular transitions between endothelial (E), hemogenic endothelial (HE), and intra-aortic hematopoietic clusters (IAHC) cells, we profiled ~6,700 cells from wildtype embryos and ~3,200 cells from endothelial Mekk3 deleted embryos by single-cell RNA sequencing (scRNA-Seq). The cells were sorted from the caudal arteries (dorsal aorta, umbilical, vitelline arteries) of embryonic day (E) 9.5 mouse embryos. We identified a developmental alteration in endothelial to hematopoietic transition (EHT) and production of HE and IAHC in endothelial Mekk3 deleted embryos. Single cell RNA-Seq profiles of cells involved in endothelial to hematopoietic transition (EHT) from wildtype and endothelial Mekk3 deleted mouse embryos at embryonic day 9.5.为探究内皮细胞Mekk3如何调控内皮细胞（endothelial，E）、生血内皮细胞（hemogenic endothelial，HE）与主动脉内造血簇（intra-aortic hematopoietic clusters，IAHC）之间的细胞及分子转化过程，我们采用单细胞RNA测序（single-cell RNA sequencing，scRNA-Seq），对约6700个野生型小鼠胚胎来源的细胞与约3200个内皮细胞Mekk3缺失小鼠胚胎来源的细胞开展了转录组分析。 上述细胞均取自胚胎发育第9.5天（embryonic day 9.5，E9.5）小鼠胚胎的尾动脉，包括背主动脉、脐动脉及卵黄动脉。 研究发现，在内皮细胞Mekk3缺失的小鼠胚胎中，内皮向造血转化（endothelial to hematopoietic transition，EHT）过程以及生血内皮细胞与主动脉内造血簇的生成均出现发育异常。 本数据集涵盖了胚胎发育第9.5天的野生型小鼠胚胎与内皮细胞Mekk3缺失小鼠胚胎中，参与内皮向造血转化（EHT）过程的细胞的单细胞RNA测序谱数据。