Genome wide analysis of rat renal gene expression at two different time points during hyperoxaluria development

We are testing the ability of spironolactone as a NADPH oxidase inhibitor during an induced dietary oxalate overload by Ethylene Glycol in Sprague Dawley rats at two different time points. We looked into the development of hyperoxaluria and crystal deposition at two different time points and into differences between hyperoxaluria and crystal induced alterations in the kidneys. Eventually, we are expecting to see the role of spironolactone as an inhibitor of NADPH oxidase which is involved in the production of reactive oxygen species (ROS) which leads to oxidative stress in the living organisms leading to a plethora of vascular diseases, hypertension, and kidney diseases.本研究旨在评估螺内酯（spironolactone）作为烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NADPH oxidase）抑制剂的功效，在经乙二醇（Ethylene Glycol）诱导建立饮食源性草酸超负荷模型的斯普拉格·道利大鼠（Sprague Dawley rats）中设置两个不同时间节点开展实验。本研究观测了两个时间节点下高草酸尿症（hyperoxaluria）的进展与晶体沉积情况，并分析了高草酸尿症与晶体诱导的肾脏病理改变之间的差异。本研究最终期望阐明螺内酯作为NADPH氧化酶抑制剂的作用机制：该酶参与活性氧（reactive oxygen species, ROS）的生成，而ROS可引发生物体内的氧化应激，进而诱发诸多血管疾病、高血压及肾脏疾病。