Genome-wide analysis of renal gene expression during hyperoxaluria development

HLP was used to induce hyperoxaluria. Hyperoxaluria will lead to crystallization and up regulation of various NADPH oxidase subunits followed by increased expression of different specific genes. It is our hypothesis that crystallization induces inflammation of the kidneys via the activation of renin-angiotensin system (RAS) and production of reactive oxygen species (ROS) through NADPH oxidase complex. Apocynin was used to block hyperoxaluria and production of reactive oxygen species (ROS) with the inhibition of NADPH oxidase system as Apocynin inhibits membrane translocation of p47 subunit of NADPH oxidase.本研究采用HLP诱导高草酸尿症。高草酸尿症可引发结晶形成，并上调多种烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NADPH oxidase）亚基的表达，进而上调多种特异性基因的表达。本研究提出如下假说：结晶可通过激活肾素-血管紧张素系统（RAS），并经由NADPH氧化酶复合物产生活性氧（ROS），从而诱发肾脏炎症。本研究使用阿波西宁（Apocynin）阻断高草酸尿症与活性氧（ROS）的生成，其作用机制为：阿波西宁可抑制NADPH氧化酶p47亚基的膜转位，进而发挥对NADPH氧化酶系统的抑制作用。