Next Generation Sequencing Analysis of miRNA expression profile in E9.5 mouse embryonic heart

Purpose: To identify miRNA expresssion profiles in E9.5 mouse embryonic heart Methods: Total RNA of E9.5 heart were extracted with TRIZOL, miRNA deep sequencing were performed in using Illumina Hiseq 2500, SE50 (RIBOBIO, http://www.ribobio.com/), producing over 10 million reads from each sample. Clean reads were mapped to mouse genome (mm9), using miRDeep2 Results: MiRNAs that were highly expressed in E9.5 embryonic heart were identified Conclusions: Results provide insight into the role of miRNAs function in E9.5 embryonic heart development Perform miRNA sequencing in wild type E9.5 mouse embryonic heart本数据集旨在鉴定E9.5小鼠胚胎心脏中的微小RNA（microRNA, miRNA）表达谱。实验方法：采用TRIZOL试剂提取E9.5小鼠心脏的总RNA，随后使用Illumina Hiseq 2500与SE50测序平台（RIBOBIO，http://www.ribobio.com/）开展miRNA深度测序，单样本可产出超1000万条读段；经质控得到的清洁读段通过miRDeep2工具比对至小鼠参考基因组mm9。实验结果：成功鉴定出在E9.5小鼠胚胎心脏中高表达的miRNA。研究结论：本研究结果为解析miRNA在E9.5小鼠胚胎心脏发育过程中的功能角色提供了重要视角。本数据集针对野生型E9.5小鼠胚胎心脏开展miRNA测序。