The genomic DNA from exponential phase cells was cross-linked and digested with 200 unit MboI enzyme (NEB) as previously described36. The cutting DNA ends were labelled with biotin-14-dCTP (TriLINK) f
This study is aimed at sequencing the Hi-C library of mulberry using Illumina technology at high depth to generate a good quality whole genome assembly.
Fresh leaves were used to construct Hi-C libraries, and the restriction endonuclease DpnII was used for DNA ligation. After tailing, pulldown, and adapter ligation, the DNA library was sequenced on an
