Fresh leaves were used to construct Hi-C libraries, and the restriction endonuclease DpnII was used for DNA ligation. After tailing, pulldown, and adapter ligation, the DNA library was sequenced on an
This study is aimed at sequencing the Hi-C library of mulberry using Illumina technology at high depth to generate a good quality whole genome assembly.
The genomic DNA from exponential phase cells was cross-linked and digested with 200 unit MboI enzyme (NEB) as previously described36. The cutting DNA ends were labelled with biotin-14-dCTP (TriLINK) f
